Alternative codon usage in E.coli

GFP was synthesized from alternative start codons (i.e. not AUG).  GFP initiated from non-standard start codon GUG became protein and folded correctly, resulting in green fluorescence!

This takes my introductory cell and molecular biology courses and puts a giant asterisk on protein synthesis.

See the full article @ NAR: https://doi.org/10.1093/nar/gkx070

For a graphic summary:
Mind blown.





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